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AIC ANUSHAKTI
AIC ANUSHAKTI
Synergizing Deep Tech

A Kit for Detection of Intracellular Iron (Fe²⁺)

Iron overload in human body, one of the most hazardous toxic conditions, has been associated with multiple diseases including neonatal liver diseases, neurodegeneration including Alzheimer’s and Parkinson’s disease, Friedreich’s Ataxia, cancer etc. Selective and sensitive detection of intracellular Fe²⁺ can lead to a better understanding of disease prognosis.

BARC has developed a simple one-step kit that contains a novel turn-on fluorescent probe that enables live-cell fluorescent imaging of intracellular Fe²⁺. The method is effortless, rapid and cost-effective, and has immense applications in cell biology and cancer research.

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SALIENT FEATURES OF TECHNOLOGY
  1. A rapid fluorometric kit for sensing Fe²⁺ in live cells.
  2. Two-step synthesis procedure from locally available materials.
  3. Kit remains stable for 12 months at 4–8°C.
  4. The methodology is rapid, simple and cost effective.
APPLICATIONS
  1. Applications in cell biology and cancer research for monitoring iron-based physiological changes.
Introduction

Iron is one of the most abundant metal on earth crust and is essential in human body...

Advantages
  1. A cost-effective fluorescence turn-on kit.
  2. Two step synthesis procedure.
  3. Stable for 24 months.
  4. No sophisticated instrumentation required.
Applications
  1. Detection of labile iron pool in live cells.
Setup Requirements

Raw Materials: Chemical raw materials available locally.

Equipments:

  1. Hot Plate with magnetic stirrer
  2. Glass reactor assembly
  3. Weighing balance
  4. Solvent distillation system
  5. Refrigerator
  6. Column chromatography

Space:

  1. Dust-free room (~25°C)
  2. Working table
  3. Water supply

Power: Three phase connection

Manpower: 1 skilled worker